Effects of Cultivation Temperature on Fatty Acid Composition in Rhodotorula gracilis.

Growth Characteristics of Rhodotorula gracilis

Previous studies have shown that Rhodotorula gracilis is capable of forming large quantities of fat under suitable conditions (Enebo et al., 1946; Pan et al., 1949; Steinberg and Ordal, 1954a). It also has been demonstrated that growth and fat formation takes place in two distinct phases (Enebo et al., 1946). Steinberg and Ordal (1954a, 1954b) have studied the fattening phase in some detail; ho...

Fatty Acid Metabolism in Serratia Marcescens. Iv. the Effect of Temperature on Fatty Acid Composition.

Effects of temperature variation on the fatty acid composition of a psychrophilic Candida species.

During the batch cultivation of a psychrophilic Candida species, the proportions of oleic and linolenic acid esters in the cells underwent a cyclic variation regardless of the incubation temperature.

Effects of pH and temperature on the fatty acid composition of bacillus acidocaldarius.

The fatty acid composition of lipid extracts from cells of Bacillus acidocaldarius grown at temperatures of 50 to 70 C and pH values of 2 to 5 was determined by gas chromatography of the methyl esters. The most abundant fatty acids are 11-cyclohexylundecanoic and 13-cyclohexyltridecanoic, followed by anteiso- and iso-heptadecanoic; unsaturated acids are absent. Highly aerated cultures produce m...

Studies on the Reaction Mechanism of Rhodotorula gracilis

We have studied D-amino-acid oxidase from Rhodotorula gracilis by site-directed mutagenesis for the purpose of determining the presence or absence of residues having a possible role in acid/base catalysis. Tyr-223, one of the very few conserved residues among D-aminoacid oxidases, has been mutated to phenylalanine and to serine. Both mutants are active catalysts in turnover with D-alanine, and ...